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exosomal protein markers cd9  (Proteintech)


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    Structured Review

    Proteintech exosomal protein markers cd9
    Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical <t>exosomal</t> markers <t>CD9,</t> CD63, and CD81, confirming vesicle identity
    Exosomal Protein Markers Cd9, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosomal protein markers cd9/product/Proteintech
    Average 96 stars, based on 520 article reviews
    exosomal protein markers cd9 - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Protective effects of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on radiation-induced epididymal cells damage via USP44/RBM14 axis"

    Article Title: Protective effects of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on radiation-induced epididymal cells damage via USP44/RBM14 axis

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-025-04782-9

    Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical exosomal markers CD9, CD63, and CD81, confirming vesicle identity
    Figure Legend Snippet: Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical exosomal markers CD9, CD63, and CD81, confirming vesicle identity

    Techniques Used: Flow Cytometry, Expressing, Staining, Transmission Assay, Electron Microscopy, Concentration Assay, Western Blot



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    Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical <t>exosomal</t> markers <t>CD9,</t> CD63, and CD81, confirming vesicle identity
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    Proteintech antibodies against exosomal markers cd9
    Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical <t>exosomal</t> markers <t>CD9,</t> CD63, and CD81, confirming vesicle identity
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    Proteintech exosome markers cd9 cd63 tsg101
    Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical <t>exosomal</t> markers <t>CD9,</t> CD63, and CD81, confirming vesicle identity
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    Proteintech exosomal markers cd9
    Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical <t>exosomal</t> markers <t>CD9,</t> CD63, and CD81, confirming vesicle identity
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    https://www.bioz.com/result/exosomal markers cd9/product/Proteintech
    Average 96 stars, based on 1 article reviews
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    Proteintech exosomal marker proteins cd 9
    miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
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    miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
    Antibodies Against Exosome Markers Cd9, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against exosome markers cd9/product/Proteintech
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    Proteintech exosomal markers
    miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
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    Image Search Results


    Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical exosomal markers CD9, CD63, and CD81, confirming vesicle identity

    Journal: Stem Cell Research & Therapy

    Article Title: Protective effects of mouse bone marrow mesenchymal stem cell-derived extracellular vesicles on radiation-induced epididymal cells damage via USP44/RBM14 axis

    doi: 10.1186/s13287-025-04782-9

    Figure Lengend Snippet: Characterization of mBMSC and Their EVs. A Flow cytometry histograms showing expression of surface markers. Cells were negative for hematopoietic markers CD14, CD19, CD34, and CD45 (< 0.5%) and positive for mesenchymal markers CD29, CD44, CD73, and CD90 (> 98%). Gray shading represents isotype controls; red lines represent specific antibody staining. B Representative images of lineage-specific staining after differentiation induction: Alizarin Red (osteogenesis), Alcian Blue (chondrogenesis), and Oil Red O (adipogenesis). Scale bars, 100 μm. C Transmission electron microscopy images of EVs showing typical morphology and size. Scale bars represent 0.5 μm (left) and 200 nm (right). D Nanoparticle tracking analysis illustrating extracellular vesicle size distribution and concentration, with a peak around 100 nm. E Western blot detection of typical exosomal markers CD9, CD63, and CD81, confirming vesicle identity

    Article Snippet: Exosomal protein markers CD9 (Proteintech, #20,597–1-AP, 1:2000), CD81 (Proteintech, #27,855–1-AP, 1:2000) and CD63 (Proteintech, #67,605–1-Ig, 1:5000) were detected by Western blotting.

    Techniques: Flow Cytometry, Expressing, Staining, Transmission Assay, Electron Microscopy, Concentration Assay, Western Blot

    miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of exosomal miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Gingipains disrupt bone homeostasis via dual regulation of osteogenesis and osteoclastogenesis through exosomal miR-146a-5p/TRAF6 signaling

    doi: 10.3389/fcimb.2025.1614126

    Figure Lengend Snippet: miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of exosomal miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.

    Article Snippet: Exosomal marker proteins CD 9 (proteintech, 20597-1-AP, 1:4000) and CD 81 (proteintech, 27855-1- AP, 1:2000) were examined by western blot.

    Techniques: In Vitro, Quantitative Proteomics, Microarray, Expressing, Transfection, Control, Software, Staining